Peggy Sue reagents and consumables
Peggy Sue reagents and consumables are designed for use on with Peggy Sue and must be used in conjunction with Compass Software version 2.5 or higher and compatible firmware.
Size Assay Modules
Part # | Description |
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DM-001 Buy Online |
Anti-Rabbit Detection Module for Jess, Wes, Peggy Sue or Sally Sue Use with a Jess/Wes or Sally Sue/Peggy Sue Separation Module. Includes: Luminol-S, Peroxide, Antibody Diluent 2, Anti-Rabbit Secondary Antibody, Streptavidin-HRP. |
DM-002 Buy Online |
Anti-Mouse Detection Module for Jess, Wes, Peggy Sue or Sally Sue Use with a Jess/Wes or Sally Sue/Peggy Sue Separation Module. Includes: Luminol-S, Peroxide, Antibody Diluent 2, Anti-Mouse Secondary Antibody, Streptavidin-HRP. |
DM-003 Buy Online |
No secondary Detection Module for Jess, Wes, Peggy Sue or Sally Sue Use with a Jess/Wes or Sally Sue/Peggy Sue Separation Module. Includes: Luminol-S, Peroxide, Antibody Diluent 2, Streptavidin-HRP. |
DM-004 Buy Online |
Biotin Detection Module for Jess, Wes, Peggy Sue or Sally Sue Use with a Jess/Wes or Sally Sue/Peggy Sue Separation Module. Includes: Secondary Streptavidin HRP, Luminol-S, Peroxide, Antibody Diluent 2, and Streptavidin-HRP (for biotinylated ladder). |
DM-005 Buy Online |
Anti-Human IgG Detection Module for Jess, Wes, Peggy Sue or Sally Sue Use with a Jess/Wes or Sally Sue/Peggy Sue Separation Module. Includes: Anti-Human IgG Secondary HRP Antibody, Luminol-S, Peroxide, Antibody Diluent 2, and Streptavidin-HRP. |
DM-006 Buy Online |
Anti-Goat Detection Module for Jess, Wes, Peggy Sue or Sally Sue Use with a Jess/Wes or Sally Sue/Peggy Sue Separation Module. Includes: Anti-Goat Secondary HRP Antibody, Luminol-S, Peroxide, Milk-free Antibody Diluent (use to dilute goat primary antibody and as blocking agent), and Streptavidin-HRP. Upon receipt, store at 2-8°C. |
DM-TP01 Buy Online |
Total Protein Detection Module for Jess, Wes, Peggy Sue or Sally Sue Use with a Jess/Wes or Sally Sue/Peggy Sue Separation Module. Includes: Luminol-S, Peroxide, Antibody Diluent 2, Total Protein Reconstitution Agent 2, Total Protein Streptavidin-HRP. |
SM-S001 Buy Online |
12-230 kDa Peggy Sue or Sally Sue Separation Module Use with a Detection Module. Reagents for 480 data points includes two parts: Separation box with 5 boxes of Capillaries (96 capillaries per box), 5 Plates, Wash Buffer, Upper and Lower Running Buffers 2, Upper and Lower Running Buffer cups, Wash Buffer cup, 5 Manifold Cleaning Sponges, Separation Matrix 2, Stacking Matrix 2, 10X Sample Buffer, plus 2 x Standard Pack 1 clamshell with 8 ea. DTT, FL Standard master mix with 29 kDa System Control protein (Store at 4°C). |
SM-S002 Buy Online |
66-440 kDa Peggy Sue or Sally Sue Separation Module Use with a Detection Module. Reagents for 480 data points includes two parts: Separation box with 5 boxes of Capillaries (96 capillaries per box), 5 Plates, Wash Buffer, Upper and Lower Running Buffers 3, Upper and Lower Running Buffer cups, Wash Buffer cup, 5 Manifold Cleaning Sponges, Separation Matrix 3, Stacking Matrix 2, 10X Sample Buffer, plus 2 x Standard Pack 3 clamshell with 8 ea. DTT, FL Standard master mix with 90 kDa System Control protein (Store at 4°C). |
SM-S003 Buy Online |
2-40 kDa Peggy Sue or Sally Sue Separation Module Requires Compass v3.0 or higher. Use with a Detection Module. Reagents for 480 data points includes two parts: Separation box with 5 boxes of Capillaries (96 capillaries per box), 5 Plates, Wash Buffer, Upper and Lower Running Buffers 4, Upper and Lower Running Buffer cups, Wash Buffer cup, 5 Manifold Cleaning Sponges, Separation Matrix 2, Stacking Matrix 2, 10X Sample Buffer, plus 2 x Standard Pack 5 clamshell with 8 ea. DTT, FL Standard master mix with 26 kDa System Control protein (Store at 4°C). |
Charge Assay Kits
Part # | Description |
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CBS2001 Buy Online |
XDR Charge Separation Master Kit for Peggy Sue, Peggy or NanoPro 1000 Includes 5 boxes of capillaries, five 384-well plates with lids, Anolyte, Catholyte, Wash Concentrate, Luminol, and Peroxide XDR and manifold cleaning sponges. |
CBS404 Buy Online |
HeLa Lysate Controls Kit - Bicine/CHAPS Buffer for Charge Assays Includes 5 vials each of low phospho and high phospho HeLa lysate, lysed in Bicine/CHAPS Lysis Buffer (5 µL/vial). Ships overnight on dry ice. Upon receipt, store components at 4°C. |
CBS403 Buy Online |
Lysis Kit - Bicine/CHAPS Buffer for Charge Assays Includes Bicine/CHAPS Lysis Buffer, Aqueous Inhibitor, DMSO Inhibitor Mix, and Cell Wash Buffer. Ships overnight on dry ice. Upon receipt, store components at 4°C. |
CBS401 Buy Online |
Lysis Kit - RIPA Buffer for Charge Assays Includes RIPA Buffer, Aqueous Inhibitor, DMSO Inhibitor Mix and Cell Wash Buffer. Ships overnight on dry ice. Upon receipt, store components at 4°C. |
041-126 Buy Online |
Amplified Rabbit Secondary Antibody Detection Kit Includes SA-HRP conjugate (50 µL), Secondary Goat-Anti-Rabbit-Biotin Conjugate (50 µL); Antibody Diluent included. REAGENT FOR PEGGY OR NANOPRO 1000 USERS ONLY. ProteinSimple strongly recommends use of the Antibody Diluent for dilution of primary and secondary antibodies as it has been proven superior to any other buffer system tested. Ships overnight at room temperature. Upon receipt, store at 4°C. |
041-127 Buy Online |
Amplified Mouse Secondary Antibody Detection Kit Includes SA-HRP conjugate (50 µL), Secondary Goat-Anti-Mouse-Biotin Conjugate (50 µL); Antibody Diluent included. REAGENT FOR PEGGY OR NANOPRO 1000 USERS ONLY. ProteinSimple strongly recommends use of the Antibody Diluent for dilution of primary and secondary antibodies as it has been proven superior to any other buffer system tested. Ships overnight at room temperature. Upon receipt, store at 4°C. |
PS-T003 Buy Online |
Peggy System Charge Training Kit Includes a XDR Charge Separation Master Kit for Peggy Sue, Peggy or NanoPro 1000 and additional reagents |
Individual Reagents
Part # | Description |
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PS-ST05EZ-8 Buy Online |
8 pk, EZ Standard Pack 5 Includes 8 packets with one each of the Single Use Biotinylated Ladder (MW ladder spans 2-40 kDa) and Single Use FL Standard (26 kDa system control protein) vials. Reagents for 200 Simple Western data points. For use with Jess, Wes, Sally Sue, or Peggy Sue. |
PS-ST04EZ-8 Buy Online |
8 pk, EZ Standard Pack 4 Includes 8 packets with one each of the Single Use Biotinylated Ladder (MW ladder spans 66-440 kDa) and Single Use FL Standard (200 kDa system control protein) vials. Reagents for 200 Simple Western data points. For use with Jess, Wes, Sally Sue, or Peggy Sue. |
PS-ST03EZ-8 Buy Online |
8 pk, EZ Standard Pack 3 Includes 8 packets with one each of the Single Use Biotinylated Ladder (MW ladder spans 66-440 kDa) and Single Use FL Standard (90 kDa system control protein) vials. Reagents for 200 Simple Western data points. For use with Jess, Wes, Sally Sue, or Peggy Sue. |
PS-ST02EZ-8 Buy Online |
8 pk, EZ Standard Pack 2 Includes 8 packets with one each of the Single Use Biotinylated Ladder (MW ladder spans 12-230 kDa) and Single Use FL Standard (180 kDa system control protein) vials. Reagents for 200 Simple Western data points. For use with Jess, Wes, Sally Sue, or Peggy Sue. |
PS-ST01EZ-8 Buy Online |
8 pk, EZ Standard Pack 1 Includes 8 packets with one each of the Single Use Biotinylated Ladder (MW ladder spans 12-230 kDa) and Single Use FL Standard (29 kDa system control protein) vials. Reagents for 200 Simple Western data points. For use with Jess, Wes, Sally Sue, or Peggy Sue. |
PS-FL01-8 Buy Online |
Fluorescent 5x Master Mix 1 Includes 8 packets with one each of the Single Use FL Standard (29 kDa system control protein) and Single Use DTT vials. For use with Jess, Wes, Peggy Sue or Sally Sue 12-230 Separation Modules |
PS-FL03-8 Buy Online |
Fluorescent 5x Master Mix 3 Includes 8 packets with one each of the Single Use FL Standard (90 kDa system control protein) and Single Use DTT vials. For use with Jess, Wes, Peggy Sue or Sally Sue 66-440 kD Separation Modules |
PS-FL05-8 Buy Online |
Fluorescent 5x Master Mix 5 Includes 8 packets with one each of the Single Use FL Standard (26 kDa system control protein) and Single Use DTT vials. For use with Jess, Wes, Peggy Sue or Sally Sue 2-40 kD Separation Modules |
043-226 Buy Online |
Peggy Sue or Sally Sue 66-440 kDa Split Running Buffer Kit Use with Compass v2.7 or higher. Includes both the Upper (043-209) and Lower (043-210) Running buffers 3. |
043-225 Buy Online |
Peggy Sue or Sally Sue 12-230 kDa Split Running Buffer Kit Use with Compass v2.7 or higher. Includes both the Upper (043-163) and Lower (043-164) Running buffers 2. |
043-326 Buy Online |
Peggy Sue or Sally Sue 2-40 kDa Split Running Buffer 4 Kit Requires Compass v3.0 or higher. Includes both the Upper (043-329) and Lower (043-330) Running buffers 4 |
042-976 Buy Online |
Total Protein Streptavidin-HRP Use with Compass v2.6 or higher. Streptavidin-HRP for Total Protein Assay for Jess/Wes, Sally Sue/Peggy Sue, 2.0 mL. Ready to use. |
042-918 Buy Online |
Peggy Sue/Sally Sue 66-440 kDa Separation Matrix Use with Compass v2.6 or higher. Separation Matrix 3 for Peggy Sue or Sally Sue (2.5 mL) |
043-328 Buy Online |
Peggy Sue/Sally Sue 2-40 kDa Separation Matrix Requires Compass v3.0 or higher. Separation Matrix 4 for Peggy Sue or Sally Sue (2.5 mL) |
042-512 Buy Online |
Separation Matrix II (2.5 mL) for Peggy Sue or Sally Sue Separation matrix II (2.5 mL) |
042-513 Buy Online |
Stacking Matrix for Peggy Sue or Sally Sue Stacking matrix II (1.5 mL) |
042-195 Buy Online |
Jess, Wes, Sally Sue, Peggy Sue Sample Buffer Jess, Wes, Sally Sue, Peggy Sue 10X Sample Buffer (440 µL) |
042-206 Buy Online |
Anti-Rabbit Secondary Antibody Goat Anti-Rabbit Secondary HRP Conjugate (ready-to-use reagent). For Jess, Wes, Sally Sue, and Peggy Sue only. |
042-205 Buy Online |
Anti-Mouse Secondary Antibody Goat Anti-Mouse Secondary HRP Conjugate (ready-to-use reagent). For Jess, Wes, Sally Sue, and Peggy Sue only. |
043-426 Buy Online |
20X Anti-Rabbit HRP Conjugate 20X Anti-Rabbit HRP Conjugate, 150 µL. To be diluted to 1X with RTU Anti-Mouse Secondary Antibody (042-205) when multiplexing with primary antibodies raised in mouse and rabbit. |
042-203 Buy Online |
Antibody Diluent 2 Antibody Diluent 2 for Jess, Wes, Peggy Sue and Sally Sue (20 mL). Ships overnight at room temperature. We strongly recommends use of the Antibody Diluent for dilution of primary and secondary antibodies as it has been proven superior to any other buffer system tested. Upon receipt, store at 4°C. |
042-196 Buy Online |
10X System Control Primary Antibody-Rabbit To be used with rabbit primary antibody to Antibody Diluent. Detects system controls. Match with Standard pack 1, 2, 3 or 4 (250 µl/vial) |
042-191 Buy Online |
10X System Control Primary Antibody-Mouse To be used with mouse primary antibody to Antibody Diluent. Detects system controls. Match with Standard pack 1, 2, 3 or 4 (250 µl/vial) |
042-518 Buy Online |
Matrix Removal Buffer for Peggy Sue or Sally Sue Matrix Removal Buffer II (140 mL) |
042-414 Buy Online |
Streptavidin-HRP Streptavidin-HRP Conjugate (ready-to-use reagent) for use with the Simple Western Size Standard. |
042-520 Buy Online |
Wash Buffer for Peggy Sue or Sally Sue Wash Buffer (170 mL) |
043-311 Buy Online |
Luminol-S Jess, Wes, Peggy Sue, Sally Sue, Peggy, Sally or Simon Luminol-S (2 mL) |
043-379 Buy Online |
Peroxide Jess, Wes, Peggy Sue, Sally Sue, Peggy, Sally or Simon Peroxide (2 mL) |
042-486 Buy Online |
Erk 1 Primary Antibody for Size Assays Erk 1 Rabbit Primary Antibody, 1X stock (1 mL) |
042-488 Buy Online |
HeLa Lysate Controls for Jess, Wes, Sally Sue and Peggy Sue HeLa lysate, lysed in RIPA Lysis Buffer (20 µL/vial). For use with Jess, Wes, Sally Sue, or Peggy Sue. |
043-522-2 Buy Online |
Anti-Goat Secondary HRP Antibody Donkey Anti-Goat Secondary HRP Conjugate (ready-to-use reagent). For Jess, Wes, Sally Sue, and Peggy Sue (2 x 1 mL). Use our Milk-free Antibody Diluent (043-524) to dilute goat primary and as a blocking agent. Ships at room temperature. Upon receipt, store at 2-8°C. |
043-491-2 Buy Online |
Anti-Human IgG Secondary HRP Antibody Goat Anti-Human Secondary HRP Conjugate (ready-to-use reagent). For Jess, Wes, Sally Sue, and Peggy Sue (2 x 1 mL). Ships at room temperature. Upon receipt, store at 2-8°C. |
043-459-2 Buy Online |
Secondary Streptavidin-HRP Secondary Streptavidin-HRP Conjugate (ready-to-use reagent). For Jess, Wes, Sally Sue, and Peggy Sue (2 x 1 mL). Use with a biotinylated antibody. For total protein assay use 042-976 and to detect biotinylated molecular weight ladders use 042-414. Ships with ice pack. Upon receipt, store at 2-8°C. |
043-524 Buy Online |
Milk-free Antibody Diluent Milk-free Antibody Diluent for Jess, Wes, Peggy Sue and Sally Sue (20 mL). We recommend its use for dilution of goat primary, anti-goat secondary and as blocking agent as it has been shown to be superior in reducing background compared to other diluents tested. Ships at room temperature. Upon receipt, store at 2-8°C. |
040-655 Buy Online |
Goat-Anti-Mouse-HRP Secondary Antibody HRP-conjugated Goat-Anti-Mouse Secondary Antibody (50 µL); Antibody Diluent included. ProteinSimple strongly recommends use of the Antibody Diluent for dilution of primary and secondary antibodies as it has been proven superior to any other buffer system tested. Ships overnight at room temperature. Upon receipt, store at 4°C. |
040-656 Buy Online |
Goat-Anti-Rabbit Secondary Antibody HRP-conjugated Goat-Anti-Rabbit Secondary Antibody (50 µL); Antibody Diluent included. ProteinSimple strongly recommends use of the Antibody Diluent for dilution of primary and secondary antibodies as it has been proven superior to any other buffer system tested. Ships overnight at room temperature. Upon receipt, store at 4°C. |
041-081 Buy Online |
Goat-Anti-Rabbit HUX HRP Secondary Antibody HRP-Conjugated Goat-Anti-Rabbit Secondary Antibody cross-absorbed for minimal cross-reactivity against human antigens (50 µL); Antibody Diluent included. ProteinSimple strongly recommends use of the Antibody Diluent for dilution of primary and secondary antibodies as it has been proven superior to any other buffer system tested. Ships overnight at room temperature. Upon receipt, store at 4°C. |
040-474 Buy Online |
Pan Erk Primary Antibody Pan Erk Primary Antibody, 1X stock (1 mL) |
040-477 Buy Online |
Phospho-Erk 1/2 Primary Antibody Phospho-Erk 1/2 Primary Antibody, 1X stock (1 mL); Ships overnight at room temperature. Upon receipt, store at 4°C. |
040-644 Buy Online |
pI Standard Ladder 1 pI Standard Ladder 1: 4.0, 4.9, 6.0, 6.4, 7.3 (175 µL); Ships overnight at room temperature. Upon receipt, store at 4°C. |
040-645 Buy Online |
pI Standard Ladder 2 pI Standard Ladder 2: 4.2, 4.9, 6.0, 6.4, 7.0 (175 µL); Ships overnight at room temperature. Upon receipt, store at 4°C. |
040-646 Buy Online |
pI Standard Ladder 3 pI Standard Ladder 3: 4.9, 6.0, 6.4, 7.0, 7.3 (175 µL); Ships overnight at room temperature. Upon receipt, store at 4°C. |
040-647 Buy Online |
pI Standard Ladder 4 pI Standard Ladder 4: 4.9, 5.5, 6.0 (175 µL); Ships overnight at room temperature. Upon receipt, store at 4°C. |
040-024 Buy Online |
pI Standard, 4.0 pI Standard, 4.0 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C. |
040-025 Buy Online |
pI Standard, 4.2 pI Standard, 4.2 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C. |
040-026 Buy Online |
pI Standard, 4.4 pI Standard, 4.4 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C. |
040-027 Buy Online |
pI Standard, 4.9 pI Standard, 4.9 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C. |
040-028 Buy Online |
pI Standard, 5.5 pI Standard, 5.5 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C. |
040-029 Buy Online |
pI Standard, 6.0 pI Standard, 6.0 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C. |
040-030 Buy Online |
pI Standard, 6.4 pI Standard, 6.4 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C. |
040-031 Buy Online |
pI Standard, 7.0 pI Standard, 7.0 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C. |
040-032 Buy Online |
pI Standard, 7.3 pI Standard, 7.3 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C. |
041-036 Buy Online |
pI Standard, 8.4 pI Standard, 8.4 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C. |
040-790 Buy Online |
pI Standard, 9.7 pI Standard, 9.7 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C. |
040-967 Buy Online |
Premix G2, ampholyte-free separation gradient Premix G2, ampholyte-free separation gradient (1.5 mL); Ships overnight at room temperature. Upon receipt, store at 4°C and in the dark. |
040-968 Buy Online |
Premix G2, pH 3–10 separation gradient Premix G2, pH 3–10 separation gradient (1.5 mL); Ships overnight at room temperature. Upon receipt, store at 4°C and in the dark. |
040-970 Buy Online |
Premix G2, pH 4–7 (nested) separation gradient Premix G2, pH 4–7 (nested) separation gradient, pH 2-4 plug (1.5 mL); Ships overnight at room temperature. Upon receipt, store at 4°C and in the dark. |
040-969 Buy Online |
Premix G2, pH 4–9 separation gradient Premix G2, pH 4–9 separation gradient (1.5 mL); Ships overnight at room temperature. Upon receipt, store at 4°C and in the dark. |
040-972 Buy Online |
Premix G2, pH 5–8 (nested) separation gradient, pH 2–4 plug Premix G2, pH 5–8 (nested) separation gradient, pH 2–4 plug (1.5 mL); Ships overnight at room temperature. Upon receipt, store at 4°C and in the dark. |
040-973 Buy Online |
Premix G2, Pharmalyte pH 5–8 separation gradient Premix G2, Pharmalyte pH 5–8 separation gradient (1.5 mL); Ships overnight at room temperature. Upon receipt, store at 4°C and in the dark. |
040-974 Buy Online |
Premix G2, Servalyt pH 5–8 separation gradient Premix G2, Servalyt pH 5–8 separation gradient (1.5 mL); Ships overnight at room temperature. Upon receipt, store at 4°C and in the dark. |
040-337 Buy Online |
Anolyte Refill Anolyte Refill (200 mL) |
040-338 Buy Online |
Catholyte Refill Catholyte Refill (200 mL) |
041-108 Buy Online |
Wash Concentrate Refill for Peggy or NanoPro 1000 Wash Concentrate Refill (200 mL). REAGENT FOR PEGGY or NANOPRO 1000 USERS ONLY. Ships overnight at room temperature. Upon receipt, store at 4°C. |
040-652 Buy Online |
Luminol Luminol (2 mL); Ships overnight at room temperature. Upon receipt, store at 4°C. |
040-483 Buy Online |
RIPA Lysis Buffer RIPA Lysis Buffer (10 mL); Ships overnight at room temperature. Upon receipt, store at 4°C. |
040-764 Buy Online |
Bicine/CHAPS Lysis Buffer and Sample Diluent Bicine/CHAPS Lysis Buffer and Sample Diluent (10 mL); Ships overnight at room temperature. Upon receipt, store at 4°C. |
040-510 Buy Online |
DMSO Inhibitor Mix DMSO Inhibitor Mix, 50X (200 µL); Ships overnight at room temperature. Upon receipt, store at 4°C. |
040-482 Buy Online |
Aqueous Inhibitor Mix Aqueous Inhibitor Mix, 25X (400 µL); Ships overnight on dry ice. Upon receipt, store at -20°C. |
040-649 Buy Online |
Sample Diluent Sample Diluent (3 mL); Ships overnight at room temperature. Upon receipt, store at 4°C. |
040-309 Buy Online |
Antibody Diluent Antibody Diluent (10 mL) |
Consumables
Part # | Description |
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SS701 Buy Online |
Capillaries - Size Separation for Peggy Sue/Sally Sue (5 pack) Peggy/Sally and Peggy Sue/Sally Sue Capillaries for size assays (5 boxes of 96 capillaries) |
SS700 Buy Online |
Capillaries - Size Separation for Peggy Sue/Sally Sue (1 pack) Peggy Sue/Sally Sue Capillaries for size assays (1 box of 96 capillaries) |
031-102 Buy Online |
Repeater Pipette for Peggy Sue or Sally Sue Rainin AR-E1 positive-displacement electronic repeater pipette (0.1 - 50 mL). Use to dispense the Separation Matrix II for Sally Sue and Peggy Sue. |
031-103 Buy Online |
Repeater Pipette 0.5 mL Tips for Peggy Sue or Sally Sue A box of 100 of the 0.5 mL syringe tips used with the Peggy Sue/Sally Sue Repeater Pipette. |
040-663 Buy Online |
Assay Plate/Lid Kit (5-Pack) for Peggy/Sally, Peggy Sue/Sally Sue, or NanoPro 1000 Includes five 384-well plates and lids. * Must be ordered with p/n 041-528. |
041-528 Buy Online |
Sponge Pack for Peggy/Sally, Peggy Sue/Sally Sue, or NanoPro 1000 Includes 6 manifold cleaning sponges. * Must be ordered with p/n 040-663. |
041-606 Buy Online |
Pipetting Guide Pipetting guide for Sally, Peggy, Sally Sue or Peggy Sue Size Assay |
041-595 Buy Online |
Instrument Cup (set of 3) Includes set of 3 instrument cups |