FluorChem E system
Do more with less
Never miss a faint band again! Every aspect of the FluorChem™ E system is optimized for superior analysis of chemiluminescent Western blots.
Intuitive Operation
Simplified touch screen control for effortless detection of chemiluminescent, colorimetric and UV fluorescent gels and blots.
Effortless Workflow
Just choose your application, capture your image, then analyze your results!
Unparalleled Performance
With imaging speeds and dynamic range that surpasses film, crucial data are not lost due to under- or overexposure.
I'm tired of doing multiple exposures with film to get the images I need
With a 5-log dynamic range enabled by the large, high-resolution CCD sensor in FluorChem imagers, you can detect faint bands and bright bands without oversaturating in a single exposure. And you get your data in minutes, with no waste at all.
My proteins are close in molecular weight, and I need to be able to differentiate between them on a single blot
The 8.3 megapixel CCD cameras in FluorChem imagers ensure that you'll get the best image quality and the resolution you need to do accurate analysis, and differentiate between bands that are close together. The FluorChem M and R also enable multiplex fluorescent imaging, letting you analyze overlapping proteins in the same blot.
I don't want anything complicated—I just want to see my proteins
Digital Darkroom software makes taking images super simple. Simply load the blot or gel, select a pre-programmed protocol on the touch-screen interface, and hit "Expose." The software automatically calculates and sets exposure and camera settings in order to acquire the best image. Once you have your image, analysis can be done on the spot in a matter of minutes. Digital Darkroom can even be accessed and controlled remotely, so you can review images and do analysis from your desk or even your phone.
We're doing a lot of different applications, and we don't want to have to buy multiple imagers
FluorChem imagers are versatile—the FluorChem R has true multi-mode capabilities, with UV fluorescence, white light, chemiluminescence, visible fluorescence, and infrared fluorescence imaging, all in one compact system. Cover all your application bases with one easy-to-use system. The FluorChem E and M can be upgraded to the R for ultimate flexibility.
I need to publish my results—how do I know I can get the quality images I need?
Our imagers have been published in over 20,000 publications worldwide. Our scientific-grade, high-resolution cameras ensure that you get great looking images that are perfect for publication.
I need to do analyses like densitometry and total protein normalization
AlphaView software, which comes with all AlphaImagers and FluorChem imagers, is packed with many advanced analysis features. From molecular weight analysis, lane profiling, and multiplex band analysis to colony counting and total protein normalization, AlphaView has everything you need for simple and complex applications.
| FEATURE | DESCRIPTION |
|---|---|
| Detector | 8.3 megapixel, scientific-grade CCD, 16-bit A/D, thermo-electrically cooled to -30 °C absolute and regulated, 5-minute cool-down time |
| Optics | 50-mm, f/1.4 motorized fixed lens |
| Light Sources |
302 or 365 nm UV transilluminator Epi-white lights Trans-white light via white light conversion screen |
| Emission Filters |
6-position motorized filter wheel 590/50 nm Orange filter for EtBr or SYPRO Orange 620/40 nm Red filter for SYPRO Red, SYPRO Ruby, or Deep Purple (optional) 537/35 nm Green filter for SYBR Green, SYBR Gold, or SYBR Safe (optional) 460/40 nm Blue filter for Hoeschst Blue (optional) |
| Cabinet |
Light-tight darkroom UV safety switch |
| Computer | Integrated computer with 10.4-inch LCD touch screen, 480 GB solid-state hard drive |
| Ports | 3 USB, 1 network |
| Accessories |
UV gel imaging screen Chemiluminescence imaging screen White light conversion screen Blue light conversion screen (optional) |
| Software | On-board Digital Darkroom for remote access, operation and analysis by any web-browser enabled computer or device. AlphaView for standalone Windows PC analysis. |
| Performance | |
| Field of View | 16 cm x 12 cm maximum |
| Dynamic Range | Up to 65,536 grayscale |
| Limit of Detection | Low femtogram |
| Specifications | |
| Data Format | 16-bit and 8-bit PNG file output |
| Size | 62 cm D x 62 cm H x 32 cm W (24.5 in x 24.5 in x 12.6 in) |
| Weight | 34 kg (75 lb) |
| Power | 100-230V AC, 345 W |
| Certifications | CE, TUV |