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Advanced Western Blotting Solutions for Neuroscience
Some of the most prevalent and least understood diseases are those that affect the nervous system. ProteinSimple offers advanced western blotting solutions including Simple Western™, Single-Cell Western, and FluorChem™ Imagers that solve common challenges associated with neuroscience protein assays and enable new insights into the biological processes that accompany neurological and neurodegenerative diseases.
Market-Leading Sensitivity, Quantitation, Multiplexing and Heterogeneity insights so you can get the most data out of your precious samples
Simple Western, Single-Cell Western and FluorChem Imagers are doing their part to win the race to discovery in neurological diseases by offering high sensitivity quantification of proteins from rare, sparingly-available samples at unprecedented speeds while also offering multiple multiplexing strategies to get the most data out of your precious neuroscience sample. With Single-Cell Westerns, you can measure heterogeneity in your samples and study protein expression within specific rare neural cell subtypes.
Stop Pooling Mice to Get Western Blotting Data. Market Leading Western Blot Sensitivity on 3 µL of Sample
Simple Western requires only 3 µL of sample while offering pg-level sensitivity, so there is no longer any need to pool samples like mouse tissue. Get single-animal statistical data and realize significant cost savings and save mouse lives. Simple Western also ups the datapoint count on laser capture microdissection samples.
To get the most data out of precious samples, Simple Western also eliminates the arduous strip and re-probe procedure by offering RePlex™, which efficiently removes the antibodies from the first round of probing for a second round of probing with fresh antibodies. The second round may also be used for Total Protein Detection, so you can normalize your data with confidence.
Total protein concentration was used as the internal loading control for protein quantification in tau transgenic mice. (a) Representative capillary electrophoresis immunoblot on cytosolic (Cyt.) and nuclear (Nuc.) enriched fractions using antibodies against Lamin A/C and b-Actin. (b) Representative capillary electrophoresis immunoblot of brain homogenates from control or tau transgenic mice using antibodies against Lamin A/C and b-Actin. (c) Representative capillary electrophoresis immunoblot used to detect total protein concentration. (d) Quantification of Lamin A/C, (e) b-Actin and (f) total protein concentration. (g) Representative electropherogram generated from capillary electrophoresis total protein immunoblot. Figure adapted with permission from Musi et al. (2018) Aging Cell. Read more about how Dr. Miranda Orr sets a new pace for Alzheimer's research
Dissociation of Mouse Neural Tissue for Single-Cell Western Analysis
In this application note, we describe and characterize a protocol to successfully dissociate mouse neural tissue micro-surgically dissected from combined cortex, ventricular zone, and hippocampus regions of E18 mice into single cells. Using a multiplexed Single-Cell Western assay to analyze protein expression in the dissociated single neuronal cells, we identify 3 subpopulations of cells within the tissue: astrocytes, radial glia, and immature neurons based on expression of their respective phenotypic protein markers: GFAP, Pax6, and β-III tubulin.
Identify and Quantify Neural Cell Subtypes with Single-Cell Western
The Milo™ Single-Cell Western platform allows you to identify and quantify the percentage of neural subtypes in your sample and track differentiation progression based on subtype-specific marker expression analysis. In this application note, we demonstrate how Milo can be used to identify and quantify neural subtypes in a heterogeneous neural sample and monitor the differentiation of induced pluripotent stem cells (iPSCs) into neurons, astrocytes, and oligodendrocytes using R&D Systems research-grade or GMP differentiation reagents.
Many scientists count on Simple Western, Single-Cell Western, and FluorChem as essential tools for the discovery and validation of biomarkers for conditions such as Parkinson’s Disease, Traumatic Brain Injury, Dementia, Alzheimer’s Disease, Multiple Sclerosis and Epilepsy. Simple Western™ assays on Jess™, Abby™, Peggy Sue™ or Sally Sue™ offer fully automated Western analysis by charge or size separation. Download our Advances in Neuroscience eBook to learn more.