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Simple Plex Assays for Viral Titer Quantification

Fast and reproducible viral capsid measurement is essential for those focused on gene transfer, as part of a cell and gene therapy workflow. While traditional plate-based and dot blot assays can be useful, they are often complex, time-consuming, and non-standardized.

Automated Simple Plex™ assays on the Ella™ platform offer a fast, sensitive, and reproducible method for reliable viral capsid measurement. Simple Plex assays are available to quantify the viral titer of both lentivirus and adeno-associated virus capsids.

  • Measure viral titer across a broad dynamic range
  • Get triplicate data results with just 25 µL or less of sample volume
  • Streamline workflow with only 15 minutes of hands-on assay prep time.
  • Achieve the same reproducible answers across multiple users and multiple sites
  • Get highly reproducible validated data with no manual steps in just 90 minutes

Scaling Analytics for Cell and Gene Therapy with Ella

Watch how Dr. Kiren Baines, a bioprocessing scientist from eXmoor, uses Ella to scale analytics for AAV process development.


To meet increased throughput and safety demands, investigators are seeking more automated analytical solutions for assessment of viral vector identity, purity, and stability. The Simple Plex AAV2 assay on the Ella platform provides an accurate and efficient method for measuring the AAV2 capsid titer. The assay uses the recombinant AAV2 antibody (A20R) from industry leader PROGEN to quantify assembled AAV2 capsids.

Combining the proven specificity of PROGEN AAV2 antibodies with the convenient workflow and robustness of the Ella platform, the Simple Plex AAV2 viral titer assay:

  • Offers a broad viral detection range combined with hands-free automation to accelerate AAV2 viral capsid quantification
  • Uses factory calibrated assay cartridges, which help minimizes setup time and reduce user-dependent variability while reducing the need for sample retests
  • Relies on the innovative Ella platform to perform every step of the immunoassay automatically, from wash steps to final
  • Provides a robust alternative to the conventional ELISA or dot blot approaches
  • Delivers superior assay sensitivity, helping to ensure data quality and consistency
Scientist using AAV viral titer assay to quantify viral titer using ELISA system Ella

Comparing the Simple Plex AAV2 Assay with the PROGEN AAV2 ELISA

In this application note, we demonstrate assay comparability between the Simple Plex AAV2 assay and the PROGEN AAV2 Titration ELISA (PRATV) to show that you get the same high-quality data you would expect from the PROGEN ELISA with an automated, streamlined workflow.

Download AAV Assay Application Note

AAV2 Assay Specification Sheet

Application note describing AAV viral titer assays on automated ELISA platform Ella

Creating Lentiviral Vectors With Fast, Reliable Viral Titration

With the growing use of lentiviral vectors derived from HIV-1 in gene transfer workflows, researchers need fast, reliable viral titer quantification during process development. The 3-to-6-hour timeframe required to run a standard ELISA is a challenge for operators who increasingly require results quickly to inform real-time decision making.

The Simple Plex assay for the detection of human HIV-1 Gag p24 combines high throughput with superior accuracy and precision to enable rapid quantification of lentiviral capsid proteins throughout different stages of cell and vector manufacturing. The automated, reproducible nature of the Simple Plex p24 assay helps eliminate user error, making it an ideal tool in good manufacturing practice (GMP) environments where quality, standardization of process, assay transfer, and scalability are highly desirable.

Download the HIV-1 GAG p24 Assay Specification Sheet


Bio-Techne offers a diverse portfolio of products and services designed to accelerate and simplify your cell and gene therapy workflow. From GMP proteins and small molecules to state-of-the-art analytical instrumentation, Bio-Techne is committed to providing high quality innovative solutions. Learn more about Bio-Techne’s cell and gene therapy offerings.

Learn About Bio-Techne Cell & Gene Therapy Solutions
Simple Plex Automated Viral Titer Assays: A Primer

What are viral titer assays?

Viral titer assays are designed to quantify viral vector concentration. In addition to cell and gene therapy gene transfer workflows, viral titer assays are integral to the study of infectious disease pathogenesis and vaccine development. At a high level, viral titer assays quantify: DNA titer, infectious titer, or capsid titer. DNA titer assays quantify the number of viral capsids containing DNA. Infectious titer assays quantify infectious particles that transfer DNA into host cells via a process called viral transduction. Capsid titer assays quantify viral capsids. Capsids may or may not contain viral DNA.

Learn more about viral titer assays at our Partner PROGEN’s website


What type of viral titer assay is the Simple Plex Immunoassay?

Simple Plex automated viral titer assays quantify viral particles, also known as capsids.

Learn more about Simple Plex Assays and the Ella Platform


I am looking for automated and scalable solutions for cell and gene therapy research and manufacturing. What do you suggest?

ProteinSimple, a Bio-techne Brand has several analytical solutions to suit your cell and gene therapy research and development needs. Download our eBook to see real-world examples of time, cost, and sample savings from our analytical solutions.

Download the Next Generation Analytical Solutions for Cell and Gene Therapy eBook  

Do you have any other resources for cell and gene therapy research and manufacturing?

Bio-Techne spans the cell and gene therapy workflow. Solutions are available for immune cell therapy and regenerative medicine. Gene engineering services are also available using our nonviral TC BusterTM gene delivery system. GMP-grade raw materials and services are also available.

Find out more about our solutions for cell and gene therapy research and manufacturing, at

Simple Plex Applications


COVID-19 Research Multiplex Assays


Inflammation Research Assays


Cancer Research Multiplex Assays


Neuroscience Research Multiplex Assays


Cell Therapy

Cell Therapy Research Multiplex Assays

Bioprocess Dev.

Bioprocess Development Multiplex Assays

Preclinical Res.

Simple Plex for Preclinical Research

Viral Titer Assay

Viral titer assays on automated ELISA system Ella
Other Simple Plex Products

Single analyate, multi-analyte, and multiplex assay options for automated ELISA system Ella

  16x1 32x1 16x2 16x3 16x4 32x2 72x1 32x3 32x4 32x5 32x6 32x7 32x8
Samples 16 32 16 16 16 32 72 32 32 32 32 32 32
Plex 1 1 2 3 4 2 1 3 4 5 6 7 8
Replicates 3 3 3 3 3 3 3 3 3 2 2 2 2
Reportable Results 16 32 32 48 64 64 72 96 128 160 192 224 256

Single Plex

Single Plex cartridge


Multianalyte cartridge


Multiplex cartridge


Customizable cartridge


Stocked cartridges